The plant genetic engineering and its application in agriculture 植物基因工程及其在農(nóng)業(yè)中的應(yīng)用
National laboratory of protein engineering and plant genetic engineering , peking university , beijing , 100871 北京大學(xué)蛋白質(zhì)及植物基因工程國(guó)家重點(diǎn)實(shí)驗(yàn)室,北京, 100871
The national laboratory of protein engineering and plant genetic engineering , college of life sciences , peking university , beijing , 100871 北京大學(xué)生命科學(xué)學(xué)院,蛋白質(zhì)工程及植物基因工程國(guó)家重點(diǎn)實(shí)驗(yàn)室,北京, 100871
Through comparison detailed information about the characteristics of the two promoters can also be obtained , which is necessary for precisely regulated gene expression in plant genetic engineering 通過(guò)對(duì)種子特異性啟動(dòng)子的比較可加深對(duì)其表達(dá)模式的了解,為植物基因工程中的精細(xì)調(diào)控提供依據(jù)。 2
The seeds of plants , especially the oil plants and corn , are related very closely to human life and , therefore , are becoming important target material for plant genetic engineering 摘要植物的種子,尤其是油料和谷類(lèi)作物的種子與人類(lèi)的生產(chǎn)生活關(guān)系非常密切,因此,也成為植物基因工程中進(jìn)行改良的重要目標(biāo)材料。
The study of the structure and function of these promoters will help not only to comprehend the development of seeds and the mechanisms of gene expression and organic substance accumulation but also search for some promoter elements useful for plant genetic engineering 對(duì)這類(lèi)啟動(dòng)子的結(jié)構(gòu)和功能元件的了解,有助于人們更加深入地理解高等植物基因表達(dá)調(diào)控機(jī)制,提高人們對(duì)植物種子發(fā)育過(guò)程及有機(jī)物在種子中積累機(jī)制的認(rèn)識(shí),而且可以為植物基因工程中生物反應(yīng)器的研究提供有應(yīng)用價(jià)值的啟動(dòng)子元件。
Abstract : the low expression level of foreign genes in transgenic plants is a puzzling question for researchers in plant genetic engineering . the biosafety concern is now causing a growing public wariness of transgenic plants around the world . this article reviews the new strategies currently used in construction of plant expression vector for plant genetic engineering . these strategies are important for obtaining better expression of foreign genes and developing safer transgenic plants 摘要外源基因在轉(zhuǎn)基因植物中表達(dá)效率低一直是令相關(guān)研究者困擾的一個(gè)問(wèn)題.轉(zhuǎn)基因植物的生物安全性最近在全球范圍內(nèi)開(kāi)始引起世人的擔(dān)憂(yōu).本文簡(jiǎn)要介紹了近年來(lái)在植物表達(dá)載體構(gòu)建方面所采用的一些新策略,這些策略有助于增強(qiáng)外源基因的表達(dá)水平、提高生物工程體的安全性。
With its broad - spectrum and non - specific potent antibacterial activity , cationic antibacterial peptide has the potential to be widely used in plant disease resistance genetic engineering . however , cema was refrained in plant genetic engineering application due to its toxic to plant cells . the modification of cema will facilitate its wide application 由于其抗菌譜廣、殺菌力強(qiáng)等優(yōu)點(diǎn),在植物抗病基因工程中有廣闊的應(yīng)用前景,但由于對(duì)植物細(xì)胞有毒害作用, cema在植物遺傳轉(zhuǎn)化中的應(yīng)用受到很大的限制。
By means of plant genetic engineering , foreign insects resistance gene can be transferred into plant cell . we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method . and obtained the transgenic mustard plants . the main results are as follows : 1 . isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves . the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers 本實(shí)驗(yàn)是利用植物基因工程獲得抗蟲(chóng)的轉(zhuǎn)基因芥菜植株,結(jié)果如下: 1豇豆胰蛋白酶抑制劑基因的分離分別提取豇豆種子、子葉及葉片的總rna ,逆轉(zhuǎn)錄成cdna 。以豇豆胰蛋白酶抑制劑基因兩端的序列為引物,用rt - pcr的熱啟動(dòng)方法從上述cdna中擴(kuò)增出目的基因片斷。